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This study conducts a thorough review of fuel cell technology, including types, economy, applications, and V2G scheme. Fuel cells have been considered for diverse applications, namely, electric vehicles, specialty vehicles such as warehouse forklifts, public transportation including buses, trains, and ferries. Other applications include grid-related, stationary, and portable applications. Among available five types of fuel cells, PEMFC is presently the optimal choice for electric vehicle usage due to its low operating temperature and durability. Meanwhile, high temperature fuel cells such as MCFC and SOFC currently remain the best choice for utility and grid related applications. The economy of fuel cells has been continuously improving and has been illustrated to only grow into a potential main source of sustainable energy soon. With the transportation sector, as fuel cell electric vehicles evolve, V2G technology is beneficial towards energy efficiency and fuel cell economy. There is evidence for V2G using FCEV being more advantageous in comparison to conventional BEVs. The costs of the five types of fuel cell vary from US$1784 to US$4500 per kW capacity. The findings are beneficial for researchers and industry professionals who wish to gain comprehensive understanding of fuel cells for adoption and development of the emerging low-emission energy solutions. 

We present an efficient, effective, and economical approach, named E3technology, for proteomics sample preparation. By immobilizing silica microparticles into the polytetrafluoroethylene matrix, we develop a robust membrane medium, which could serve as a reliable platform to generate proteomics-friendly samples in a rapid and low-cost fashion. We benchmark its performance using different formats and demonstrate them with a variety of sample types of varied complexity, quantity, and volume. Our data suggest that E3technology provides proteome-wide identification and quantitation performance equivalent or superior to many existing methods. We further propose an enhanced single-vessel approach, named E4technology, which performs on-filter in-cell digestion with minimal sample loss and high sensitivity, enabling low-input and low-cell proteomics. Lastly, we utilized the above technologies to investigate RNA-binding proteins and profile the intact bacterial cell proteome. 

Abstract BackgroundThe maize inbred line A188 is an attractive model for elucidation of gene function and improvement due to its high embryogenic capacity and many contrasting traits to the first maize reference genome, B73, and other elite lines. The lack of a genome assembly of A188 limits its use as a model for functional studies. ResultsHere, we present a chromosome-level genome assembly of A188 using long reads and optical maps. Comparison of A188 with B73 using both whole-genome alignments and read depths from sequencing reads identify approximately 1.1 Gb of syntenic sequences as well as extensive structural variation, including a 1.8-Mb duplication containing the Gametophyte factor1 locus for unilateral cross-incompatibility, and six inversions of 0.7 Mb or greater. Increased copy number of carotenoid cleavage dioxygenase 1 (ccd1) in A188 is associated with elevated expression during seed development. Highccd1expression in seeds together with low expression of yellow endosperm 1 (y1) reduces carotenoid accumulation, accounting for the white seed phenotype of A188. Furthermore, transcriptome and epigenome analyses reveal enhanced expression of defense pathways and altered DNA methylation patterns of the embryonic callus. ConclusionsThe A188 genome assembly provides a high-resolution sequence for a complex genome species and a foundational resource for analyses of genome variation and gene function in maize. The genome, in comparison to B73, contains extensive intra-species structural variations and other genetic differences. Expression and network analyses identify discrete profiles for embryonic callus and other tissues.